The SideroTec™-Total Assay is the first in a range of kits for Siderophore detection. SideroTec™-Total is an easy-to-use colorimetric test that facilitates rapid detection of all siderophores and other synthetic iron chelation molecules. With ready-to-use, stable reagents, this means minimal set up is required, with results available in 15 minutes.
The SideroTec-Total kits contains all the reagents required for Siderophore detection with sufficient materials to run up to 110 tests. It can be used for both qualitative as well as quantitative assay and can be used either manually or automated for high throughput screening. With minimal set up and rapid assay time, the SideroTec-Total assay kit is a convenient and cost-effective method for siderophore detection.
Accuplex are building a range of assay kits for siderophores detection and detection of other iron chelation molecules. Siderophores are low molecular weight compounds released by most bacteria and fungi that serve primarily to transport iron into the microorganism.
Iron is an essential element necessary for the growth and survival of the microorganism. In a low iron environment, microbes generate and secrete siderophores which then scavenge for free iron. The siderophore chelates iron from the surrounding environment and the chelated iron-siderophore complex is then reabsorbed by the microbe enabling it to grow and proliferate.
Siderophores offer targets for multiple applications including novel biomarkers of infection, iron chelation molecules to treat iron overload in thalassemia or hemochromatosis, as well as several other applications.
As siderophores come in several different types, Accuplex will supply a range of tests that facilitate total siderophore detection as well as identify the type of siderophore.
The SideroTecTM-Hydroxamate assay kit is a simple one step rapid assay that facilitates identification of the hydroxamate type siderophores produced by a microorganism. Reagents are ready-to-use and enable results within 5 minutes.
The SideroTec™-Catecholate assay kit completes the set of rapid assay kits that help identify and classify catecholate siderophore generated by a micro-organism. The assay provides reagents in a ready-to-use simple three step assay format, with results available in 5 minutes.
“I do think the kit would be of interest to many in the siderophore community, especially if it is quantitative and has a decent dynamic range.”Glaxo Smith Kline
“Overall, we are very pleased with its sensitivity, rapid analysis time and its ease of use. We have tried many times to make the siderophore assay solution from literature recipes without much success-certainly not the sensitivity of your assay.”American Type Culture Collection
“We have run a few of our chelators using your kit and found it useful for ranking the compounds in order of increasing iron binding affinity at pH 7.4. Overall the assay is much faster and more convenient than what we usually do.”Apopharma
“Your assay gave the precise concentration of the siderophores produced by our reference strain (mutant of Mycobacterium smegmatis). This was confirmed by HPLC. We had positive feed-back on the siderophore work and detection kit during ESBES.”IBB - Institute for Biotechnology and BioengineeringPortugal